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Interscapular brown adipose tissue was homogenized in Tris-ethylenediaminetetraacetic acid (EDTA) buffer (10 mM Tris and 1 mM EDTA, pH 7.4) with 1% protease inhibitor (Nacalai Tesque, Kyoto, Japan) on ice

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Ahmed, A., Wong, R

The cells were cultivated in Dulbeccos Modified Eagle Medium (DMEM) with high glucose, 10% fetal bovine serum (FBS), and 1% penicillin-streptomycin, at 37 C in a humidified atmosphere containing 5% CO