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Whatever your protein source is, youll want to load it onto the column in pretty much the same way cap the bottom, add your interacting protein or cell lysate, cap the top, and incubate with gentle agitation for around an hour

10.3390/ijms22084130 73 StefanescuR.Tero-VescanA.NegroiuA.AuricE.VariC

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