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Cell membranes were solubilized by 0.5% (w/v) LMNG (Anatrace, Maumee, USA) supplemented with 0.1% (w/v) CHS (Anatrace) with 5 M GLP-2 and Protease Inhibitor Cocktail (Topscience) at 4 C for 2 h, followed by centrifugation at 65,000 g for 30 min at 4 C

High TMAO levels were found to induce activation of the nuclear factor-kappa B (NF-B) pathway and increase the expression of proinflammatory genes including those encoding inflammatory cytokines, adhesion molecules, and chemokines (72)