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Reconstituted Retatrutide should be refrigerated at 36-46 degrees Fahrenheit (2-8 degrees Celsius)

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After an overnight fasting, an upper abdominal midline incision was made under anesthesia (100 mg/kg ketamine and 10 mg/kg xylazine, intraperitoneally, i.p.)

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Participants with other diseases which could influence biological indicators were excluded, such as diabetes mellitus, hypertension, hyperthyroidism, hyperlipidemia, alcohol abuse, rheumatoid arthritis, or tumor