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At 5,000 mcg/mL, 250 mcg = 0.05 mL = 5 units

10.1016/j.tibs.2019.03.005 617 TurzoS.SeffernickJ

Conversely, when adding water to the powder vial, you may need to let some air escape into the syringe so the pressure doesnt push the plunger back out