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The total RNA from the animals of each group was pooled together and cDNA was prepared using reverse transcriptase enzyme (Thermo Fischer, USA

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doi: 10.1016/j.jplph.2007.06.008 73 NadasenT.Transient expression of two Cercospora zeina fungal effectors in Nicotiana benthamiana MSc Biotechnology dissertation (2022).Pretoria, South Africa: University of Pretoria

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& Nielsen, J