A FACS Canto II flow cytometer (BD Biosciences) was used for the analysis employing Kaluza software (Beckman Coulter, Brea, CA, USA)
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We further explored the induction of ER stress by measuring additional ER stress markers such as calreticulin, using Western blotting
Peptide purity is rarely the issue execution precision determines whether protocols achieve documented 58% lean mass gains or fail to produce measurable results
Original research by Derosa, et al
Statements Data availability statement The original contributions presented in the study are included in the article/supplementary material, further inquiries can be directed to the corresponding author
