(11)Fmoc-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (11) Preparation of Fmoc-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (10)Fmoc-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin(20DMF)5203DMF3DMF2Fmoc-Pro-OH0.8gDIEA0.4mlTBTU0.56gHOBT0.2gDMF10ml1.53DMF3DMF2Fmoc-Pro-Gly-Lys(Boc)-Pro- Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin In step (10) of Fmoc-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin, add decapping reagent (20% piperidine in DMF solution), reacted at room temperature for 5 minutes, drained, added decapping agent and reacted at room temperature for 20 minutes, washed 3 times with isopropanol, washed 3 times with DMF, washed 2 times with re-distilled DMF, drained, added Fmoc-Pro- 0.8g of OH, 0.4ml of DIEA, 0.56g of TBTU, 0.2g of HOBT, and 10ml of DMF were reacted at room temperature for 1.5 hours, dried with a vacuum pump, washed 3 times with isopropanol, washed 3 times with DMF, washed 2 times with distilled DMF, pumped Dry, obtain Fmoc-Pro-Gly-Lys (Boc)-Pro-Ala-Asp (otBu)-Asp (otBu)-Ala-Gly-Leu-Val-WangResin
This is what I do
Do not disregard professional medical advice or delay in seeking professional advice because of something you have read in this article.
Brito A, Verdugo R, Hertrampf E, Miller JW, Green R, Fedosov SN, Shahab-Ferdows S, Sanchez H, Albala C, Castillo JL et al
Pharmacol Rev 41:143210 Corpas FJ, Barroso JB, Carreras A, Valderrama R, Palma JM, Len AM, Sandalio LM, del Ro LA (2006) Constitutive arginine-dependent nitric oxide synthase activity in different organs of pea seedlings during plant development
[DOI] [PubMed] [Google Scholar] 34.Dallenne C, Da Costa A, Decre D
