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In the First set, we vary the concentration of GSH (05 M) by keeping the concentration constant of another substrate constant ( Figure 4A ), whereas in the next set we vary the concentration of H 2 O 2 (01000 M) by keeping the concentration There were no changes in the amounts of nanospheres, NADPH (200 M), and GR (5 units) in the both set ( Figure 4B )