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Detection of the effect of CRAT on PGC-1 expression by qRT-PCR and Western blot analysis showed that the expression of PGC-1 at protein level, but not mRNA level, was markedly decreased by CRAT overexpression and increased by CRAT knockdown (Fig

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Interestingly, LPS-induced phosphorylation of both IB and NF-B was strongly inhibited in cells treated with NAC-S2, thereby suppressing downstream cytokine production of TNF-