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If natural remedies aren't enough, you may consider using over-the-counter products like stool softeners (e.g., docusate sodium) or mild laxatives (e.g., polyethylene glycol)

Materials and methods Cell lines and reagents The MCF-7 human breast cancer cell line was obtained from Dr Dean Edwards (University of Texas, San Antonio, TX, USA) and was maintained in phenol red RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 100 U/mL penicillin, 100 g/mL streptomycin, 1 non-essential amino acids and bovine insulin at 6 ng/mL

[ [PMID: 29766634] et al

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Conventional proteases like trypsin often provide insufficient cleavage due to limited recognition sites or steric hindrance from bulky modifications