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Liver regeneration is significantly impaired in TGR5 KO mice compared to wild-type controls, highlighting the protective role of TGR5 in this process

We examined glutamine metabolism in mouse embryonic fibroblasts (MEFs) isolated from mice that have triple knock-outs (TKO) of all three Rb family members (Rb-1, Rbl1 and Rbl2) and found that loss of global Rb function caused a marked increase in 13 C-glutamine uptake and incorporation into glutamate and tricarboxylic acid cycle (TCA) intermediates in part via upregulated expression of the glutamine transporter ASCT2 and the activity of glutaminase 1 (GLS1)

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Because non-targeted, comprehensive data have been very scarce in the literature (particularly for RBCs) 6 , we chose that approach to metabolites in fission yeast and blood