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administration 58, 59, 60, 61 of Ang II produced dose-dependent decreases in total renal blood flow (RBF) and renal cortical blood flow (CBF), and increases in renal vascular resistance and renal cortical vascular resistance, which were blocked by AT 1 receptor antagonists

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Factor 4: Room-temperature injection Injecting refrigerated solution increases pain reports by 19% compared to room-temperature solution

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These results indicate that APR-246 induces p53-independent effects in these cells and that these off-target effects prevent us from detecting any mutant-specific effects even at low doses