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Upon completion of the reaction, the suspension was filtered and the filtrate rotor-evaporated (30 C

Abstract Glucocorticoids modulate the secretion of growth hormone (GH) by various and competing effects on the hypothalamus and pituitary gland

Tan M, Yin Y, Ma X, Zhang J, Pan W, Tan M, Zhao Y, Yang T, Jiang T, Li H

and Garrett, Q

Hypotheses 143:109862

The eluted proteins were subjected to size-exclusion chromatography (SEC) with a Superose 6 Increase 10/300 GL column (Cytiva) in the final buffer containing 25 mM HEPES pH 7.5, 150 mM NaCl and 1 mM DTT (buffer A, for biochemical assays) or 5 mM DTT (buffer B, for cryo-EM)