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Coupled steady-state kinetic measurements with GSSR and GSH revealed that both half-reactions usually occur with second order rate constants around 10 5 to 10 6 M 1 s 1 with the reductive half-reaction being slower for wild-type glutaredoxins 11,14,15,17,19

(1999) identified this novel protein, Kelch-like ECH-associated protein1 (KEAP1), as the cytosolic negative regulator of NRF2 activity

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It's the most common and practical route, and once you've done it a few times it becomes second nature