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Dysregulation in the FXR and TGR5 signaling pathways leads to hepatocyte toxicity due to an increase in primary bile acids like cholic acid (CA) and chenodeoxycholic acid (CDCA), as well as a decrease in secondary bile acids such as deoxycholic acid (DCA) and lithocholic acid (LCA), which in turn triggers microbiota dysbiosis and MASLD pathogenesis [249]