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For a multi-use compound like IGF-1 LR3, where you'll be drawing from the same vial for multiple experiments over days or weeks, the choice is clear

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link Canapp SO Jr, et al

Abstract: The thymus gland is a central lymphoid organ in which developing T cell precursors, known as thymocytes, undergo differentiation into distinct type of mature T cells, ultimately migrating to the periphery where they exert specialized effector functions and orchestrate the immune responses against tumor cells, pathogens and self-antigens

Escalation is never automatic it requires an explicit go-ahead