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Samples were mixed with rehydration buffer (9 M urea, 2% Chaps, 0.5% immobilized pH gradient (IPG) buffer pH 310 NL, 65 mM DTT and a trace of bromophenol blue) and incubated for 30 min at room temperature

Hemodynamic stresses induce endothelial dysfunction and remodeling of pulmonary artery in experimental compensated heart failurePubMed

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