To verify that the SAT1 disruption cassettes were successfully excised from GDH2/gdh2 and GDH3/gdh3 strains, genomic PCR was used with primers C1 and C4 to amplify the region between the upper end of 5 flank sequences to the lower end of 3 flank sequences to make sure that both alleles of each gene were removed (Supplementary Fig
Rose NR, Bonita R, Burek CL (2002) Iodine: an environmental trigger of thyroiditis
The other authors report no conflicts of interest relevant to this article
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