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Loss of MLKL ameliorates liver fibrosis by inhibiting hepatocyte necroptosis and hepatic stellate cell activation

The pellet was resuspended in 200 l of 8 M urea with 10 mM Tris (pH 8), incubated at 60 C for 30 min with shaking and centrifuged for 15 min at 18,000 g for protein quantification of the supernatant with the DC Protein Assay (Bio-Rad Laboratories)

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Dissociation of the G subunit from G i , which would normally inhibit cAMP accumulation through adenylyl cyclase, switches to a stimulatory role due to the activation induced by the nearby G S activity