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The B16 parental cell line was derived from a C57BL/6 mouse which spontaneously developed lesions and were subsequently adapted to grow in cultured conditions ( GRM1 ) in melanocytes which is a useful model to study spontaneous metastasis in a biologically and physiologically relevant manner ( in vivo setting using crosses of our mice, this new strain will be a useful tool to study spontaneous metastasis ( in vivo takes much longer than the commonly used intravenous inoculation of tumor cells, in addition animal wellness rules in almost all institutions, frequently discourages the practice to keeping tumor-bearing mice for a long period of time

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NTBI exhibits high redox reactivity, leading to oxidative damage in various cells and organs [32]