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2.11 Enzyme-linked immunosorbent assay (ELISA) for cytokine quantification The levels of pro-inflammatory cytokines TNF- and IL-1 in the culture supernatant of SH-SY5Y cells were quantified using ELISA kits (R&D Systems, Inc., Minneapolis, United States)

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Over the course of the next 3 to 5 years several groups demonstrated the correctness of this hypothesis by demonstrating that MTC produces calcitonin (9,10) and that measurement of CT could be used to diagnose MTC (11)

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