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Briefly, bacterial pellets were lysed in buffer (150 mM NaCl 2 , 50 mM Tris, 10% (v/v) glycerol, 1 mM phenylmethylsulfonyl fluoride (PMSF) at pH 7.4 by bead-beating as described for the ATP assay

Because this is a small-molecule salt rather than a peptide, the usual peptide precautions about mechanical shear and methionine or tryptophan oxidation do not apply

Injections go directly into the bloodstream, allowing for better and faster absorption

Saline contains sodium chloride, whereas bacteriostatic water contains only sterile water and benzyl alcohol

In addition to antihypertensive activity, tripeptides such as GHK-Cu have been reported to contribute to vascular repair following injury

It is especially advantageous to people suffering from CFS (chronic fatigue syndrome), since it reduces the severity of symptoms