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The kit works well, and I would recommend a total loading amount of 2g protein of lysate for good performance

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C-met binding was examined at two HGF-doses (250 ng/ml and 500 ng/ml in PBS) by sandwich ELISA-like assay on recombinant c-met-Fc chimera as in Figure 1e, and significant changes in optical absorbance subtracted by a baseline level (negative control without HGF, bar 1 ) were depicted in Figure 2a (left column)

These biomarkers can be used in adjunct to MTT assay for a more accurate prediction of the in vivo response