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Just before adding it to the culture medium, include 2 mM phenylmethylsulfonyl fluoride and 2X protease inhibitors

Sometimes this issue is fixed simply by optimizing buffers, for instance by increasing salt or detergent concentration, so that the interacting protein no longer binds to the GST only control, but still binds to your GST-fusion protein of interest

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Note: if you are suffering from a medical condition it might be that you can receive this treatment from your NHS GP

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