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doi: 10.1089/ars.2010.3114 47 NordbergJArnrES

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NAD+ levels decline by approximately 50% between ages 40 and 60, as measured by Zhu et al

T cells need glutathione to grow and low glutathione can make these immune system cells less effective

To construct the sorting vector pLKO.1-GST DAF for shRNA gene knockdown experiment, we amplified the coding sequence of N-signal-GST-EGFP-GPI DAF from the pEGFP-GST-GPI DAF plasmid and joined it with FastDigest KpnI/FastDigest BamHI (Thermo Fisher) linearized pLKO.1 vector using ClonExpress II One Step Cloning Kit

Table 2 Genes relevant to cell cycle regulation upregulated by GHK Table 3 Genes relevant to stem cell function upregulated by GHK Table 4 Gene relevant to stem cells function suppressed by GHK 4