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After incubation with the secondary antibody, the cells were washed with PBST and mounted using a Fluoroshield Mounting Medium with DAPI (Abcam ab104139)

Aug 2020;146(2):367-376 e3

Asadbegi M, Yaghmaei P, Salehi I, Ebrahim-Habibi A, Komaki A (2016) Neuroprotective effects of metformin against A-mediated inhibition of long-term potentiation in rats fed a high-fat diet

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When leg muscle mass declines, as can happen on GLP-1 therapy without adequate protein or exercise, insulin sensitivity can paradoxically worsen even as body weight falls